Sasori had wide greyish brown eyes which he inherited from his mother, and short, mousy red hair which he inherited from his father. He was usually seen with a hazy, dreamy look to his appearance. As a child, Sasori wore dark green robes with a pale poncho around them. As a teenager, while living in Sunagakure, he wore a purplish robe with the Sunagakure forehead protector around his forehead. After leaving the village and conversing into a puppet body in the likeness of his original body, Sasori's hair became slightly more messy, and wore his Akatsuki robe along with a purple Akatsuki ring on his left thumb that bears the kanji for "jewel" or "sphere" (玉, gyoku). He also wore teal nail polish on his fingernails. Even though Sasori still possessed his Sunagakure forehead protector that was slashed out in the middle, he kept it on his person instead of wearing it.

Sasori's puppet body consisted of a pair of spinning claws attached to his lower back, a holder for four scrolls on his back, a stinger in the empty and open stomach cavity, a compartment in his right chest, and a 'core of living flesh' (生身の核, namami no kaku) in the left which had the kanji for "scorpion" (蠍, sasori) painted on it. In his exposed state, Sasori's eyes would also be wide open in a maniacal way, as opposed to his normal calm looking eyes.

After Kabuto reincarnated him, Sasori displayed the black sclerae and darker irides seen in other reincarnated shinobi. In place of his previous clothing, Sasori, like the other reincarnated Akatsuki members, wore a maroon cloak with beige lining. Despite being 35 years old at the time of death, his reincarnated form takes that of his teenage years, how he looked before he converted himself.